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Addgene

pAID-EF1a linearizing in pX330
(Plasmid #140610)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140610 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330
  • Backbone size w/o insert (bp) 8500
  • Total vector size (bp) 8519
  • Modifications to backbone
    sgRNA scaffold has some changes
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA for pAID-EF1a plasmid digestion
  • Insert Size (bp)
    21

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pX330 plasmid was obtained from Addgene (#42230).

Please visit https://www.biorxiv.org/content/10.1101/2020.01.20.912113v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAID-EF1a linearizing in pX330 was a gift from Tatsuo Fukagawa (Addgene plasmid # 140610 ; http://n2t.net/addgene:140610 ; RRID:Addgene_140610)

  • For your References section:

    A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nishimura K, Yamada R, Hagihara S, Iwasaki R, Uchida N, Kamura T, Takahashi K, Torii KU, Fukagawa T. Nucleic Acids Res. 2020 Oct 9;48(18):e108. doi: 10.1093/nar/gkaa748. 10.1093/nar/gkaa748 PubMed 32941625
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