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Addgene

pGEX-4T3-p85beta
(Plasmid #1406)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1406 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T3
  • Backbone size w/o insert (bp) 4950
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PI3K regulatory subunit p85beta
  • Alt name
    p85 beta
  • Alt name
    Pik3r2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2200
  • Entrez Gene
    Pik3r2 (a.k.a. p85beta)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SacII (destroyed during cloning)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cantley lab recommends growth at room temperature.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T3-p85beta was a gift from Lewis Cantley (Addgene plasmid # 1406 ; http://n2t.net/addgene:1406 ; RRID:Addgene_1406)
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