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Addgene

pB18cmBFP1A5
(Plasmid #14035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14035 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD18-Cm
  • Backbone size w/o insert (bp) 6026
  • Vector type
    Bacterial Expression ; Yeast/Bacteria optimized codons

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BFP1A5
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer See map
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB18cmBFP1A5 was a gift from Patrick Daugherty (Addgene plasmid # 14035 ; http://n2t.net/addgene:14035 ; RRID:Addgene_14035)

  • For your References section:

    Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library. Mena MA, Treynor TP, Mayo SL, Daugherty PS. Nat Biotechnol. 2006 Dec . 24(12):1569-71. 10.1038/nbt1264 PubMed 17115054
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