pCDF_LuxR
(Plasmid
#140039)
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PurposeConstutitive expression of LuxR protein.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140039 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDF
- Total vector size (bp) 3379
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin and Streptomycin, 50 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepLuxL and LuxR
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SpeciesSynthetic
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Insert Size (bp)923
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer NNNNggtaccACCTGTACGATCCTACAGGT
- 3′ sequencing primer CTGTCCGTACTTCAAAAATTAATAAGGATCCNNNN (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/849711v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDF_LuxR was a gift from Yolanda Schaerli (Addgene plasmid # 140039 ; http://n2t.net/addgene:140039 ; RRID:Addgene_140039) -
For your References section:
Controlling spatiotemporal pattern formation in a concentration gradient with a synthetic toggle switch. Barbier I, Perez-Carrasco R, Schaerli Y. Mol Syst Biol. 2020 Jun;16(6):e9361. doi: 10.15252/msb.20199361. 10.15252/msb.20199361 PubMed 32529808