XLone-Puro NFIL3 P2A eGFP NLS
(Plasmid #140026)

• Purpose: Tunable and temporal expression control of NFIL3 and nuclear eGFP
• Depositing Lab: Xiaoping Bao
• Publication: Xlone Puro plasmids for inducible gene expression in mammal cells (unpublished)

Backbone

• Vector backbone: Addgene #140027
• Backbone size w/o insert (bp): 5760
• Total vector size (bp): 7952
• Modifications to backbone: eGFP switched to NFIL3-P2A-eGFP NLS
• Vector type: Mammalian Expression, Synthetic Biology
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NFIL3 and enhanced Green Fluorescent Protein
• Alt name: E4BP4 and eGFP
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2196
• Entrez Gene: NFIL3 (a.k.a. E4BP4, IL3BP1, NF-IL3A, NFIL3A)
• Promoter: TRE3G
• Tag / Fusion Protein:
  • eGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NheI or SpeI (not destroyed)
• 5′ sequencing primer: M13 FWD
• 3′ sequencing primer: M13 RVS

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry