pAS_4xEcoLeuT(CUA)_AnapRS
(Plasmid #140019)

• Purpose: Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of azido-phenylalanine; for transient or stable piggyBac-mediated integration
• Depositing Lab: Simon Elsaesser
• Publication: Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6.

Backbone

• Vector backbone: pAS
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EcoLeuRS (AnapRS)
• Alt name: 4xh7SK LeuTyrT(CUA)
• Species: Escherichia coli
• Insert Size (bp): 2592
• Mutation: L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L538S, F541C, A560V
• Promoter: EF1

Cloning Information

• Cloning method: Ligation Independent Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS and depositors sanger sequencing can only confirm 3x EcoLeuT(CUA) repeats. Depositor however confirms plasmid is functional. We recommend additional diagnostic tests to confirm there are 4 tRNA repeats.