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Addgene

pSL3287
(Plasmid #139750)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139750 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RSF1010
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For CRISPR interference in Synechococcus sp. UTEX 2973 or other strains. Desired gRNA or gRNAs are inserted by AarI digestion of this plasmid followed by ligation.

Please not there are 3 mutations in RepC-E107D, C108A, H109M. Depositor confirms this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL3287 was a gift from Himadri Pakrasi (Addgene plasmid # 139750 ; http://n2t.net/addgene:139750 ; RRID:Addgene_139750)

  • For your References section:

    Tunable Repression of Key Photosynthetic Processes Using Cas12a CRISPR Interference in the Fast-Growing Cyanobacterium Synechococcus sp. UTEX 2973. Knoot CJ, Biswas S, Pakrasi HB. ACS Synth Biol. 2020 Jan 17;9(1):132-143. doi: 10.1021/acssynbio.9b00417. Epub 2019 Dec 24. 10.1021/acssynbio.9b00417 PubMed 31829621
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