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Addgene

RCAS(A) cNkx2.5 (CT#800)
(Plasmid #13951)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13951 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCASBP(A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nkx2.5
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    900
  • Entrez Gene
    NKX2-5 (a.k.a. CNKX-2.5, NKX-2.5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Drucilla Roberts

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Entire coding region of Nkx5.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS(A) cNkx2.5 (CT#800) was a gift from Cliff Tabin (Addgene plasmid # 13951 ; http://n2t.net/addgene:13951 ; RRID:Addgene_13951)

  • For your References section:

    Roles of BMP signaling and Nkx2.5 in patterning at the chick midgut-foregut boundary. Smith DM, Nielsen C, Tabin CJ, Roberts DJ. Development. 2000 Sep . 127(17):3671-81. PubMed 10934012
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