pBS cPtc2 (CT#4)
(Plasmid
#13942)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13942 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS SK-
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePtc2
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Alt namePatched 2
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SpeciesG. gallus (chicken)
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Insert Size (bp)1420
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Entrez GenePTCH2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
1.42kb partial cDNA insert was obtained from a chick limb bud library that was screened with a mouse ptc-2 probe from ontogeny.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS cPtc2 (CT#4) was a gift from Cliff Tabin (Addgene plasmid # 13942 ; http://n2t.net/addgene:13942 ; RRID:Addgene_13942) -
For your References section:
Ptc1 and Ptc2 transcripts provide distinct readouts of Hedgehog signaling activity during chick embryogenesis. Pearse RV, Vogan KJ, Tabin CJ. Dev Biol. 2001 Nov 1. 239(1):15-29. 10.1006/dbio.2001.0430 PubMed 11784016
Map uploaded by the depositor.
