pGEM beta catenin (CT#593)
(Plasmid
#13938)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13938 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T Easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3015
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta catenin
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SpeciesG. gallus (chicken)
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Insert Size (bp)650
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MutationAmino acids 64-272
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Entrez GeneCTNNB1 (a.k.a. CHBCAT)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site SacII (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
RT-PCR fragment corresponding to AA 64-272 of chicken beta-catenin gene according to Lu et al., 1997.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM beta catenin (CT#593) was a gift from Cliff Tabin (Addgene plasmid # 13938 ; http://n2t.net/addgene:13938 ; RRID:Addgene_13938) -
For your References section:
Dual roles of Wnt signaling during chondrogenesis in the chicken limb. Hartmann C, Tabin CJ. Development. 2000 Jul . 127(14):3141-59. PubMed 10862751