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Addgene

RCAS(A) mWnt5a (CT#539)
(Plasmid #13936)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCASBP 2A
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Wnt-5a
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Wnt5a (a.k.a. 8030457G12Rik, Wnt-5a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer RCAS-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-legth ORF of mWnt-5a;
5’ NcoI-primer : CATGACCATG GCCATGAAGC CCATTGGAAT ATTAA
3’BamHI-primer: CGGGATCCTA TTTGCACACG AACTGATCC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS(A) mWnt5a (CT#539) was a gift from Cliff Tabin (Addgene plasmid # 13936 ; http://n2t.net/addgene:13936 ; RRID:Addgene_13936)

  • For your References section:

    Dual roles of Wnt signaling during chondrogenesis in the chicken limb. Hartmann C, Tabin CJ. Development. 2000 Jul . 127(14):3141-59. PubMed 10862751
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