UGPC
(Plasmid
#138954)
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Purpose(Empty Backbone) Lentiviral vector for co-expressing Cre recombinase and sgRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 138954 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
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Vector typeMammalian Expression, Lentiviral, Cre/Lox
- Promoter U6 and PGK
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer GACTATCATATGCTTACCG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UGPC was a gift from Nadya Dimitrova (Addgene plasmid # 138954 ; http://n2t.net/addgene:138954 ; RRID:Addgene_138954) -
For your References section:
p53 Activates the Long Noncoding RNA Pvt1b to Inhibit Myc and Suppress Tumorigenesis. Olivero CE, Martinez-Terroba E, Zimmer J, Liao C, Tesfaye E, Hooshdaran N, Schofield JA, Bendor J, Fang D, Simon MD, Zamudio JR, Dimitrova N. Mol Cell. 2020 Feb 20;77(4):761-774.e8. doi: 10.1016/j.molcel.2019.12.014. Epub 2020 Jan 20. 10.1016/j.molcel.2019.12.014 PubMed 31973890