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Addgene

pLM-AMA18.0 dCas9-VPR
(Plasmid #138945)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138945 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDSM-JAK-109
  • Backbone manufacturer
    DSM
  • Backbone size w/o insert (bp) 10170
  • Modifications to backbone
    pGpda:DsRed-SKL removed
  • Vector type
    CRISPR, Synthetic Biology ; AMA1 autonomously replicating fungal plasmid
  • Selectable markers
    Fungal selection: Terbinafine (ergA) / Phleomycin/Bleomycin (ble)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
  • Species
    Synthetic; Fungal expression, Penicillium rubens
  • Insert Size (bp)
    21950
  • Promoter p40S (AN0465) Aspergillus nidulans
  • Tag / Fusion Protein
    • NLS

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLM-AMA18.0 dCas9-VPR was a gift from Arnold Driessen (Addgene plasmid # 138945 ; http://n2t.net/addgene:138945 ; RRID:Addgene_138945)

  • For your References section:

    CRISPR-based transcriptional activation tool for silent genes in filamentous fungi. Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen AJM. Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. 10.1038/s41598-020-80864-3 PubMed 33441979
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