pLM-AMA18.0 dCas9-VPR
(Plasmid #138945)

• Purpose: Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers
• Depositing Lab: Arnold Driessen
• Publication: Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3.

Backbone

• Vector backbone: pDSM-JAK-109
• Backbone manufacturer: DSM
• Backbone size w/o insert (bp): 10170
• Modifications to backbone: pGpda:DsRed-SKL removed
• Vector type: CRISPR, Synthetic Biology ; AMA1 autonomously replicating fungal plasmid
• Selectable markers: Fungal selection: Terbinafine (ergA) / Phleomycin/Bleomycin (ble)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
• Species: Synthetic; Fungal expression, Penicillium rubens
• Insert Size (bp): 21950
• Promoter: p40S (AN0465) Aspergillus nidulans
• Tag / Fusion Protein:
  • NLS

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pLM-AMA18.0 dCas9-VPR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry