pGEX Su(fu) (CT#99)
(Plasmid #13856)

• Depositing Lab: Cliff Tabin
• Publication: Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

Backbone

• Vector backbone: pGEX-KG
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Su(fu)
• Alt name: suppressor of fused
• Species: G. gallus (chicken)
• Insert Size (bp): 2000
• Mutation: Nco I fragment from full length; ~2kbp- ATG to stop plus 3’ UTR.
• Entrez Gene: SUFU
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: pGEX 5'
• 3′ sequencing primer: pGEX 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The GST–Su(fu) construct was generated by using the bacterial expression vector pGEX-KG to fuse glutathione S-transferase in-frame with a 2.0-kb NcoI chick Su(fu) fragment extending from the initial methionine (the ATG in the 5' NcoI site) to 600 bp past the stop codon.