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Addgene

pGEMHE-NLS-Beta-catenin-S33Y
(Plasmid #138537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEMHE
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 3064
  • Total vector size (bp) 5514
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beta-catenin
  • Alt name
    Ctnnb1
  • Alt name
    Cadherin associated protein, beta 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2450
  • Mutation
    changed serine 33 to tyrosine
  • GenBank ID
    NM_001098209.2
  • Promoter T7
  • Tags / Fusion Proteins
    • 2XFLAG (C terminal on insert)
    • 2xNLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Age1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene Cat#19286

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMHE-NLS-Beta-catenin-S33Y was a gift from Carmen Williams (Addgene plasmid # 138537 ; http://n2t.net/addgene:138537 ; RRID:Addgene_138537)

  • For your References section:

    Developmentally Programmed Tankyrase Activity Upregulates beta-Catenin and Licenses Progression of Embryonic Genome Activation. Gambini A, Stein P, Savy V, Grow EJ, Papas BN, Zhang Y, Kenan AC, Padilla-Banks E, Cairns BR, Williams CJ. Dev Cell. 2020 Jun 8;53(5):545-560.e7. doi: 10.1016/j.devcel.2020.04.018. Epub 2020 May 21. 10.1016/j.devcel.2020.04.018 PubMed 32442396
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