pBFv-U6.2B
(Plasmid
#138401)
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PurposegRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138401 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescriptII SK (+)
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Vector typeInsect Expression
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Selectable markersvermilion
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameU6-2
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBFv-U6.2B was a gift from Shu Kondo (Addgene plasmid # 138401 ; http://n2t.net/addgene:138401 ; RRID:Addgene_138401) -
For your References section:
Highly improved gene targeting by germline-specific Cas9 expression in Drosophila. Kondo S, Ueda R. Genetics. 2013 Nov;195(3):715-21. doi: 10.1534/genetics.113.156737. Epub 2013 Sep 3. 10.1534/genetics.113.156737 PubMed 24002648
