SIRT1 Flag
(Plasmid #13812)

• Purpose: Mammalian expression of human SIRT1 with flag tag
• Depositing Lab: Eric Verdin
• Publication: North et al Mol Cell. 2003 Feb . 11(2):437-44.

Backbone

• Vector backbone: pcDNA3.1 +
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SIRT1
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: N-terminus truncated
• Entrez Gene: SIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)
• Tag / Fusion Protein:
  • flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Roy Frye
• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).