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Bradley Human CRISPR Poison Exon Knockout Library
(Pooled Library #138084)

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  • Purpose

    The human pgPoison library targets 3` splice sites with paired gRNAs (pgRNAs), inducing skipping of "poison" cassette exons and corresponding upstream constitutive exons.

    This allows direct comparison between loss of a constitutive coding exon (knockout) with loss of a poison exon.

  • Vector Backbone

    lentiGuide-Puro (Plasmid #52963) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 138084 gRNA pooled library† 1 $380 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lentiCas9-Blast (Addgene #52962), Lenti-iCas9-neo (Addgene #85400), or any other plasmid(s) or cell lines expressing Cas9.

Library Details

  • Species
    Human
  • Exons targeted
    963
  • Paired gRNAs
    9,508
  • Controls
    1,000 non-targeting pgRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human CRISPR Poison Exon Knockout Library was a gift from Robert Bradley (Addgene #138084)

  • For your References section:

    RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exons. Thomas JD, Polaski JT, Feng Q, De Neef EJ, Hoppe ER, McSharry MV, Pangallo J, Gabel AM, Belleville AE, Watson J, Nkinsi NT, Berger AH, Bradley RK. Nat Genet. 2020 Jan;52(1):84-94. doi: 10.1038/s41588-019-0555-z. PubMed 31911676
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