Skip to main content
Addgene

pAAV.hSynap.iAChSnFR
(Plasmid #137950)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137950 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart
AAV1 137950-AAV1 Virus (100 µL at titer ≥ 7×10¹² vg/mL) and Plasmid. $405
Add to Cart

Backbone

  • Vector backbone
    pAAV.hSynap
  • Backbone size w/o insert (bp) 4373
  • Total vector size (bp) 6200
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    iAChSnFR
  • Species
    Synthetic
  • Insert Size (bp)
    1827
  • Promoter hSynanpsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CAG CGC TGC CTC AGT CTG CGG TGG GCA
  • 3′ sequencing primer GCG TAT CCA CAT AGC GTA AAA GGA GCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Information for AAV1 (Catalog # 137950-AAV1) ( Back to top)

Purpose

Ready-to-use AAV1 particles produced from pAAV.hSynap.iAChSnFR (#137950). In addition to the viral particles, you will also receive purified pAAV.hSynap.iAChSnFR plasmid DNA.

Synapsin-driven, iAChSnFR acetylcholine sensor. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume 100 µL
  • Titer ≥ 7×10¹² vg/mL
  • Pricing $375 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • Storage Store at -80℃. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids encode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
  • Buffer PBS + 0.001% Poloxamer 188 + 200 mM NaCl
  • Serotype AAV1
  • Purification Iodixanol gradient ultracentrifugation

Biosafety

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Terms and Licenses

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). The specific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV.hSynap.iAChSnFR was a gift from Loren Looger (Addgene plasmid # 137950 ; http://n2t.net/addgene:137950 ; RRID:Addgene_137950) For viral preps, please replace (Addgene plasmid # 137950) in the above sentence with: (Addgene viral prep # 137950-AAV1)

  • For your References section:

    A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies. Borden PM, Zhang P, Shivange AV, Marvin JS, Cichon J, Dan C, Podgorski K, Figueiredo A, Novak O, Tanimoto M, Shigetomi E, Lobas MA, Kim H, Zhu PK, Zhang Y, Zheng WS, Fan C, Wang G, Xiang B, Gan L, Zhang G, Guo K, Lin L, Cai Y, Yee AG, Aggarwal A, Ford CP, Rees DC, Dietrich D, Khakh BS, Dittman JS, Gan W, Koyama M, Jayaraman V, Cheer JF, Lester HA, Zhu JJ, Looger LL. bioRxiv 2020 10.1101/2020.02.07.939504
Related items:
Commonly requested with: