SIRT5.1
(Plasmid
#13738)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13738 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-KG
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSIRT5
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SpeciesH. sapiens (human)
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Entrez GeneSIRT5 (a.k.a. SIR2L5)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SphI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full length SIRT5.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SIRT5.1 was a gift from John Denu (Addgene plasmid # 13738 ; http://n2t.net/addgene:13738 ; RRID:Addgene_13738) -
For your References section:
Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases. Hallows WC, Lee S, Denu JM. Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5. 10.1073/pnas.0604392103 PubMed 16790548