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Addgene

pGL2399
(Plasmid #137051)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137051 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST
  • Vector type
    Leishmania Ribosomal Locus Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Split Cre Recombinase Rapamycin Inducible Dimerisation
  • Alt name
    DiCre

Gene/Insert 2

  • Gene/Insert name
    Blasticidin-S Deaminase

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2399 was a gift from Jeremy Mottram (Addgene plasmid # 137051 ; http://n2t.net/addgene:137051 ; RRID:Addgene_137051)

  • For your References section:

    DiCre-Based Inducible Disruption of Leishmania Genes. Duncan SM, Myburgh E, Alves-Ferreira EV, Mottram JC. Methods Mol Biol. 2019;1971:211-224. doi: 10.1007/978-1-4939-9210-2_10. 10.1007/978-1-4939-9210-2_10 PubMed 30980305
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