CMV-mMaroon-KIX-mMaroon
(Plasmid #137005)

• Purpose: mMaroon-KIX acceptor for R-CREB
• Depositing Lab: Ryohei Yasuda
• Publication: Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)31037-2. doi: 10.1016/j.neuron.2019.11.028.

Backbone

• Vector backbone: EGFP-C1
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 5700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KIX domain
• Species: R. norvegicus (rat)
• Insert Size (bp): 220
• GenBank ID: crebbp
• Entrez Gene: Crebbp (a.k.a. CBP, RSTS, RTS)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mMaroon (N terminal on insert)
  • mMaroon (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV_For

Resource Information

• A portion of this plasmid was derived from a plasmid made by: KIX domain was a gift from Oliver Griesbeck

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry