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Addgene

CMV-mEGFP-CREB (S133A)
(Plasmid #137002)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137002 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EGFP-C1
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CREB1
  • Species
    R. norvegicus (rat)
  • Mutation
    Changes Serine 133 into Alanine
  • Entrez Gene
    Creb1 (a.k.a. Creb)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    CREB was a gift from Marc Montminy, Addgene #22394

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-mEGFP-CREB (S133A) was a gift from Ryohei Yasuda (Addgene plasmid # 137002 ; http://n2t.net/addgene:137002 ; RRID:Addgene_137002)

  • For your References section:

    In Vivo Imaging of the Coupling between Neuronal and CREB Activity in the Mouse Brain. Laviv T, Scholl B, Parra-Bueno P, Foote B, Zhang C, Yan L, Hayano Y, Chu J, Yasuda R. Neuron. 2019 Dec 17. pii: S0896-6273(19)31037-2. doi: 10.1016/j.neuron.2019.11.028. 10.1016/j.neuron.2019.11.028 PubMed 31883788
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