pcDNA3-sPDGFRalpha-Fc
(Plasmid #136438)

• Purpose: Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
• Depositing Lab: Erik Procko
• Publication: Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 10.1371/journal.ppat.1008647. eCollection 2020 Jun.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5332
• Total vector size (bp): 7621
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PDGFRalpha
• Alt name: PDGFRA
• Alt name: platelet-derived growth factor receptor alpha
• Species: H. sapiens (human)
• Insert Size (bp): 2289
• Mutation: Extracellular domain of PDGFR-alpha (a.a. 1-524)
• GenBank ID: NP_006197.1
• Entrez Gene: PDGFRA (a.k.a. CD140A, PDGFR-2, PDGFR2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Linker GGGS (C terminal on insert)
  • Fc region of IgG1 (a.a. Asp-221 to Lys-447) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-fwd
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry