pSIE1
(Plasmid #136355)

• Purpose: Selection by Inducible Effectors
• Depositing Lab: Andrew Goodman
• Publication: Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB.00544-19. doi: 10.1128/JB.00544-19. Print 2020 Jan 15.

Backbone

• Vector backbone: pExchange_tdk
• Backbone size w/o insert (bp): 2980
• Total vector size (bp): 6196
• Vector type: Unspecified
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Type VI effector bfe1
• Species: Bacteroides fragilis 638R
• Insert Size (bp): 993
• Mutation: ATG removed to add signal sequence before gene
• Promoter: P1TDP-GHO23

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: --

Gene/Insert 2

• Gene/Insert name: Type VI effector bte1
• Species: Bacteroides fragilis NCTC9343
• Insert Size (bp): 864
• Mutation: ATG removed to add signal sequence before gene
• Promoter: P1TDP-GHO23

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: --

Gene/Insert 3

• Gene/Insert name: BT_4676
• Alt name: Periplasmic signal sequence
• Species: Bacteroides thetaiotaomicron VPI5482
• Insert Size (bp): 63

Gene/Insert 4

• Gene/Insert name: tetR
• Species: E. coli
• Insert Size (bp): 624

Resource Information

• Supplemental Documents:
  • Annotated Depositor's Sequence
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry