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Addgene

pLV 4xSTAR-sTomato-NLS-blast
(Plasmid #136259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size w/o insert (bp) 6256
  • Total vector size (bp) 8383
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    stem cell Ascl2 reporter, 4 repeats
  • Alt name
    4x STAR
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    350
  • Tag / Fusion Protein
    • sTomato-NLS

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note Addgene identified an IS4 transposable element in this plasmid. The IS4 element however does not disrupt any elements and the depositor has confirmed it does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV 4xSTAR-sTomato-NLS-blast was a gift from Hugo Snippert (Addgene plasmid # 136259 ; http://n2t.net/addgene:136259 ; RRID:Addgene_136259)

  • For your References section:

    Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids. Heinz MC, Oost KC, Snippert HJG. STAR Protoc. 2020 Oct 8;1(3):100126. doi: 10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18. 10.1016/j.xpro.2020.100126 PubMed 33377020
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