pLV 4xSTAR-mTFP-NLS-blast
(Plasmid #136257)

• Purpose: Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
• Depositing Lab: Hugo Snippert
• Publication: Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi: 10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18.

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size w/o insert (bp): 6256
• Total vector size (bp): 8392
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: stem cell Ascl2 reporter, 4 repeats
• Alt name: 4x STAR
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 350
• Tag / Fusion Protein:
  • mTFP-NLS

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note Addgene identified an IS4 transposable element in this plasmid. The IS4 element however does not disrupt any elements and the depositor has confirmed it does not affect plasmid function.