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Addgene

Tol2 8xSTAR-mNeonGreen. PGK-puro
(Plasmid #136255)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136255 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pminiTol2, Addgene #31829
  • Backbone manufacturer
    Stephen Ekker lab
  • Backbone size w/o insert (bp) 3498
  • Total vector size (bp) 6360
  • Vector type
    Mammalian Expression ; Tol2 transposase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    stem cell Ascl2 reporter, 8 repeats
  • Alt name
    8x STAR
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    540
  • Tag / Fusion Protein
    • mNeonGreen

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2 8xSTAR-mNeonGreen. PGK-puro was a gift from Hugo Snippert (Addgene plasmid # 136255 ; http://n2t.net/addgene:136255 ; RRID:Addgene_136255)

  • For your References section:

    Specific Labeling of Stem Cell Activity in Human Colorectal Organoids Using an ASCL2-Responsive Minigene. Oost KC, van Voorthuijsen L, Fumagalli A, Lindeboom RGH, Sprangers J, Omerzu M, Rodriguez-Colman MJ, Heinz MC, Verlaan-Klink I, Maurice MM, Burgering BMT, van Rheenen J, Vermeulen M, Snippert HJG. Cell Rep. 2018 Feb 6;22(6):1600-1614. doi: 10.1016/j.celrep.2018.01.033. 10.1016/j.celrep.2018.01.033 PubMed 29425513
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