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Addgene

pMal-EcPhr
(Plasmid #136244)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136244 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMalc2
  • Backbone manufacturer
    NEB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EcPhr
  • Species
    E. coli
  • Mutation
    V115E- Please see depositor comments below
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms V115E mutation in EcPhr does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMal-EcPhr was a gift from Aziz Sancar (Addgene plasmid # 136244 ; http://n2t.net/addgene:136244 ; RRID:Addgene_136244)

  • For your References section:

    A cryptochrome/photolyase class of enzymes with single-stranded DNA-specific photolyase activity. Selby CP, Sancar A. Proc Natl Acad Sci U S A. 2006 Nov 21. 103(47):17696-700. 10.1073/pnas.0607993103 PubMed 17062752
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