pPS1889
(Plasmid
#1361)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1361 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS316
- Backbone size w/o insert (bp) 4887
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNLS-ECFP-EYFP
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Alt namepRS316-NLS-ECFP-EYFP
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Alt nameECFP
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Alt nameEYFP
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SpeciesNA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ADH1 promoter, SV40 T-antigen NLS, ECFP, EYFP, NUF2 3' UTR, CEN plasmid
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS1889 was a gift from Pamela Silver (Addgene plasmid # 1361 ; http://n2t.net/addgene:1361 ; RRID:Addgene_1361) -
For your References section:
Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex. Damelin M, Silver PA. Mol Cell 2000 Jan;5(1):133-40. 10.1016/S1097-2765(00)80409-8 PubMed 10678175