pGGEV_4'_+nls-mCherry-Flag-_+1_BK+
(Plasmid
#49313)
-
PurposeGolden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 49313 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBluescript
-
Backbone manufacturerStratagene
-
Vector typecloning vector
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namenls-mCherry-Flag
-
SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGGEV_4'_+nls-mCherry-Flag-_+1_BK+ was a gift from Joachim Wittbrodt (Addgene plasmid # 49313 ; http://n2t.net/addgene:49313 ; RRID:Addgene_49313) -
For your References section:
Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. Kirchmaier S, Lust K, Wittbrodt J. PLoS One. 2013 Oct 7;8(10):e76117. doi: 10.1371/journal.pone.0076117. 10.1371/journal.pone.0076117 PubMed 24116091