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Addgene

PLKO.1-UPF1-3'UTR
(Plasmid #136036)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PLKO.1
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UPF1
  • gRNA/shRNA sequence
    TTATTACCCAGAATAAGATGC
  • Species
    H. sapiens (human)
  • Entrez Gene
    UPF1 (a.k.a. HUPF1, NORF1, RENT1, UTF, pNORF1, smg-2)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLKO.1-UPF1-3'UTR was a gift from Anthony Leung (Addgene plasmid # 136036 ; http://n2t.net/addgene:136036 ; RRID:Addgene_136036)

  • For your References section:

    Structure-Mediated RNA Decay by UPF1 and G3BP1. Fischer JW, Busa VF, Shao Y, Leung AKL. Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi: 10.1016/j.molcel.2020.01.021. Epub 2020 Feb 3. 10.1016/j.molcel.2020.01.021 PubMed 32017897
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