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Addgene

rescue plasmid
(Plasmid #135971)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4FLAG
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rescue plasmid was a gift from Yongming Wang (Addgene plasmid # 135971 ; http://n2t.net/addgene:135971 ; RRID:Addgene_135971)

  • For your References section:

    krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells. Wang B, Wang Z, Wang D, Zhang B, Ong SG, Li M, Yu W, Wang Y. J Biol Eng. 2019 Apr 24;13:35. doi: 10.1186/s13036-019-0150-y. eCollection 2019. 10.1186/s13036-019-0150-y PubMed 31049076
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