pCS2+8 Flag2AUF1(p40)
(Plasmid
#135945)
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PurposeExpresses AUF1p40 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 135945 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+8
- Backbone size w/o insert (bp) 4134
- Total vector size (bp) 5117
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameheterogeneous nuclear ribonucleoprotein D
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Alt nameAUF1 p40
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SpeciesH. sapiens (human)
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Insert Size (bp)983
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GenBank IDNM_002138.3
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Entrez GeneHNRNPD (a.k.a. AUF1, AUF1A, HNRPD, P37, hnRNPD0)
- Promoter CMV and SP6
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Tag
/ Fusion Protein
- Flag2 (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV forward or SP6 forward
- 3′ sequencing primer SV40 reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+8 Flag2AUF1(p40) was a gift from Yimon Aye (Addgene plasmid # 135945 ; http://n2t.net/addgene:135945 ; RRID:Addgene_135945) -
For your References section:
Post-transcriptional regulation of Nrf2-mRNA by the mRNA-binding proteins HuR and AUF1. Poganik JR, Long MJC, Disare MT, Liu X, Chang SH, Hla T, Aye Y. FASEB J. 2019 Nov 1:fj201901930R. doi: 10.1096/fj.201901930R. 10.1096/fj.201901930R PubMed 31665914