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Addgene

pCS2+8 Flag2AUF1(p40)
(Plasmid #135945)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135945 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+8
  • Backbone size w/o insert (bp) 4134
  • Total vector size (bp) 5117
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    heterogeneous nuclear ribonucleoprotein D
  • Alt name
    AUF1 p40
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    983
  • GenBank ID
    NM_002138.3
  • Entrez Gene
    HNRNPD (a.k.a. AUF1, AUF1A, HNRPD, P37, hnRNPD0)
  • Promoter CMV and SP6
  • Tag / Fusion Protein
    • Flag2 (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV forward or SP6 forward
  • 3′ sequencing primer SV40 reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+8 Flag2AUF1(p40) was a gift from Yimon Aye (Addgene plasmid # 135945 ; http://n2t.net/addgene:135945 ; RRID:Addgene_135945)
  • For your References section:

    Post-transcriptional regulation of Nrf2-mRNA by the mRNA-binding proteins HuR and AUF1. Poganik JR, Long MJC, Disare MT, Liu X, Chang SH, Hla T, Aye Y. FASEB J. 2019 Nov 1:fj201901930R. doi: 10.1096/fj.201901930R. 10.1096/fj.201901930R PubMed 31665914