integrin beta6 777T pBabe puro
(Plasmid
#13583)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 13583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBabe puro
-
Backbone manufacturerAvailable at Addgene (plasmid #1764)
- Backbone size w/o insert (bp) 5169
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameintegrin beta 6 777T
-
Alt nameITGB6
-
Alt nameintegrin beta 6
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2400
-
Mutation777T. Lacks last 11 amino acids of cytoplasmic domain.
-
GenBank IDNM_000888
-
Entrez GeneITGB6 (a.k.a. AI1H)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI/SnaBI (destroyed during cloning)
- 3′ cloning site XbaI/SnaBI (destroyed during cloning)
- 5′ sequencing primer pBabe 5' (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map for additional restriction sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
integrin beta6 777T pBabe puro was a gift from Dean Sheppard (Addgene plasmid # 13583 ; http://n2t.net/addgene:13583 ; RRID:Addgene_13583) -
For your References section:
Loss of integrin alpha(v)beta6-mediated TGF-beta activation causes Mmp12-dependent emphysema. Morris DG, Huang X, Kaminski N, Wang Y, Shapiro SD, Dolganov G, Glick A, Sheppard D. Nature. 2003 Mar 13. 422(6928):169-73. 10.1038/nature01413 PubMed 12634787
Map uploaded by the depositor.