pTrc99-Rat FABP2
(Plasmid
#13578)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13578 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTrc99
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Backbone manufacturerPharmacia
- Backbone size w/o insert (bp) 4000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameintestinal fatty acid binding protein
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Alt nameFABP2
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SpeciesR. norvegicus (rat)
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Insert Size (bp)564
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GenBank IDNM_000134
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Entrez GeneFabp2 (a.k.a. FABP)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer pBAD-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTrc99-Rat FABP2 was a gift from Martin Scanlon (Addgene plasmid # 13578 ; http://n2t.net/addgene:13578 ; RRID:Addgene_13578) -
For your References section:
The interaction of lipophilic drugs with intestinal fatty acid-binding protein. Velkov T, Chuang S, Wielens J, Sakellaris H, Charman WN, Porter CJ, Scanlon MJ. J Biol Chem. 2005 May 6. 280(18):17769-76. 10.1074/jbc.M410193200 PubMed 15722357