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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13574 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTrcHisA
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 4400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468)
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Alt namePPARalpha
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SpeciesH. sapiens (human)
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Insert Size (bp)1119
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Mutationdeleted residues 1-100.
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GenBank IDNP_005027.2
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Entrez GenePPARA (a.k.a. NR1C1, PPAR, PPAR-alpha, PPARalpha, hPPAR)
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Tag
/ Fusion Protein
- x6His N-terminal, enterokinase cleavage site
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer F1 CCAGAACAAAGAAGCTG from 495 to 500; F2 GACCTGAAATCTCTGGC
- 3′ sequencing primer CAGCAGGGTAACCTGGTC from 616 to 633 (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains an unidentified ~2.5kb band when run on an agarose gel. It is unknown whether this extra band affects plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTrcHisA-PPARalpha(aa101-468) was a gift from Martin Scanlon (Addgene plasmid # 13574 ; http://n2t.net/addgene:13574 ; RRID:Addgene_13574)