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Addgene

pTrcHisA-PPARalpha(aa101-468) Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid #13574 with the enzymes KpnI-HF and BamHI-HF

We expected the following banding patterns:

KpnI-HF single digest: 1 band at 5.5kb
BamHI-HF single digest: 1 band at 5.5kb
KpnI-HF / BamHI-HF double digest: 2 bands (1.1kb and 4.4kb)