pAAV-Sox2-CreERT2
(Plasmid #135618)

• Purpose: Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus
• Depositing Lab: Lin He
• Publication: Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e4. doi: 10.1016/j.celrep.2019.05.103.

Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Addgene 61591
• Backbone size w/o insert (bp): 3883
• Total vector size (bp): 6947
• Modifications to backbone: Contains a 981 bp stuffer sequence 3' of the insert
• Vector type: Mouse Targeting, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: P2A-CreERT2 flanked by Sox2 homology arms
• Alt name: Sox2
• Species: M. musculus (mouse)
• Insert Size (bp): 3064
• GenBank ID: 20674
• Entrez Gene: Sox2 (a.k.a. Sox-2, lcc, ysb)
• Tag / Fusion Protein:
  • P2A-CreERT2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pAAV-Sox2-CreERT2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry