pET-28a-His6-SUMO-Munc18-1wt
(Plasmid #135550)

• Purpose: Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin
• Depositing Lab: Jingshi Shen
• Publication: Yu et al Methods Mol Biol. 2019;1860:237-249. doi: 10.1007/978-1-4939-8760-3_15.

Backbone

• Vector backbone: pET28a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Munc18-1wt
• Species: R. norvegicus (rat)
• Insert Size (bp): 1782
• Mutation: No ATG start codon
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • 6xHis tag, thrombin recognition and cleavage site, then ubiquitin-like protein tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglI (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry