pAAV-syn-FLEX-jYCaMP1
(Plasmid
#135422)
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PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 5143
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namejYCaMP1
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SpeciesSynthetic
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Insert Size (bp)1261
- Promoter hSyn
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer atgcTATACGAAGTTATACTAGTGCTAGCGGATCCTACTTCGCTGTCATCATttgtacaaactc
- 3′ sequencing primer TACTAGTGGTACCACCGGTGATATCgccaccATGGTCGACTCATCACG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGENIE Project, Janelia Research Campus
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-syn-FLEX-jYCaMP1 was a gift from Kaspar Podgorski (Addgene plasmid # 135422 ; http://n2t.net/addgene:135422 ; RRID:Addgene_135422) -
For your References section:
jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths. Mohr MA, Bushey D, Aggarwal A, Marvin JS, Kim JJ, Marquez EJ, Liang Y, Patel R, Macklin JJ, Lee CY, Tsang A, Tsegaye G, Ahrens AM, Chen JL, Kim DS, Wong AM, Looger LL, Schreiter ER, Podgorski K. Nat Methods. 2020 May 25. pii: 10.1038/s41592-020-0835-7. doi: 10.1038/s41592-020-0835-7. 10.1038/s41592-020-0835-7 PubMed 32451475
Map uploaded by the depositor.
