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pcDNA3.1-hRARβ.hRXRα
(Plasmid #135415)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135415 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 8185
  • Modifications to backbone
    Neomycin gene was removed and replaced with RXRα at the sites of SmaI/BstBI.
  • Vector type
    Mammalian Expression
  • Selectable markers
    None

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Follow the protocol from Promega.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Human retinoic acid receptor-beta
  • Alt name
    RARβ
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1368
  • GenBank ID
    NM_001290216
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer ATGCTAGCGCCACCATGACCACCAGCGGCCACG
  • 3′ sequencing primer TTAAGCTTATTGCACGAGTGGTGACTG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Human retinoid X receptor-alpha
  • Alt name
    RXRα
  • Insert Size (bp)
    1389
  • GenBank ID
    NM_002957
  • Promoter SV40

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site BstB1 (not destroyed)
  • 5′ sequencing primer GCCACCATGGACACCAAACATTTCCTG
  • 3′ sequencing primer TATTCGAACTAAGTCATTTGGTGCGGCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RARβ was cloned in the multiple cloning site at NheI/HindIII. Neomycin gene was removed and replaced with RXRα at the sites of SmaI/BstBI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-hRARβ.hRXRα was a gift from Catharine Ross (Addgene plasmid # 135415 ; http://n2t.net/addgene:135415 ; RRID:Addgene_135415)

  • For your References section:

    CYP26A1 gene promoter is a useful tool for reporting RAR-mediated retinoid activity. Zolfaghari R, Mattie FJ, Wei CH, Chisholm DR, Whiting A, Ross AC. Anal Biochem. 2019 Jul 15;577:98-109. doi: 10.1016/j.ab.2019.04.022. Epub 2019 Apr 27. 10.1016/j.ab.2019.04.022 PubMed 31039331
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