pOO2-GW
(Plasmid #135097)

• Purpose: (Empty Backbone) Gateway vector for expression of proteins in Xenopus oocytes
• Depositing Lab: Wolf Frommer
• Publication: Chen et al Nature. 2010 Nov 25;468(7323):527-32. doi: 10.1038/nature09606.

Backbone

• Vector backbone: pOO2-GW
• Backbone size (bp): 5288
• Vector type: bacterial expression vector for generating SP6 transcripts in vitro for Xenopus oocyte transfection
• Promoter: SP6

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Resource Information

• Supplemental Documents:
  • p002-GW-update-annotation.gb
• A portion of this plasmid was derived from a plasmid made by: This plasmid was generated by Dominique Loque in my lab, he used a precursor generated by Uwe Ludewig in my lab. The original backbone was derived from peter Ruppertsberg at Tübingen University.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The AtAMT1;1 coding sequence with the att sites (attB1 and attB2) from the pDRf1-AtAMT1;1 (Loque et al 2007 Nature 446 195-198) was remobilized with both restriction enzymes SpeI and XhoI then subsequently cloned between SpeI and XhoI sites in the p6013-002 (Ludewig et al., 2002 J. Biol. Chem. 277, 13548-13555). The AtAMT1;1 coding sequence was then replaced by the Gateway DNA cassette after a BP reaction with pDONR 221 (GATEWAY technology, Invitrogen) to generate the p002-GW vector.