pFL-EGFP-3xFlag-DEST
(Plasmid #134903)

• Purpose: (Empty Backbone) Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag
• Depositing Lab: Andrew Deans
• Publication: Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi: 10.1016/j.celrep.2022.111749.

Backbone

• Vector backbone: pFastbac-Dual
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 5100
• Modifications to backbone: insertion of EGFP and gateway cassette for separate expression of EGFP (from p10 promoter) and 3x-Flag tagged target protein in baculovirus expression system
• Vector type: Insect Expression
• Promoter: polyhedron
• Selectable markers: Gentamycin
• Tag / Fusion Protein:
  • 3x-Flag (N terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Also contains gentamcyin resistance gene that is transferred to Baculovirus genome using DH10Bac transformation.
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: pH
• 3′ sequencing primer: SV40polyA

Resource Information

• Supplemental Documents:
  • pFL-EGFP-3xFlag-DEST.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry