pCAT202
(Plasmid
#134879)
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PurposeBacterial expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134879 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBBR1MCS-2
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemRFP1
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SpeciesSynthetic
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Insert Size (bp)900
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Mutationdeletion of Y208
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Single deletion in mRFP1 Y208 does not affect plasmid function.
Plasmid parts were assembled using Golden gate method
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAT202 was a gift from Thomas Howard (Addgene plasmid # 134879 ; http://n2t.net/addgene:134879 ; RRID:Addgene_134879) -
For your References section:
pCAT vectors overcome inefficient electroporation of Cupriavidus necator H16. Azubuike CC, Gatehouse AMR, Howard TP. N Biotechnol. 2021 Nov 25;65:20-30. doi: 10.1016/j.nbt.2021.07.003. Epub 2021 Jul 29. 10.1016/j.nbt.2021.07.003 PubMed 34333160
