pCAG-Myc-Oct3/4-IP
(Plasmid #13460)

• Depositing Lab: Shinya Yamanaka
• Publication: Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9.

Backbone

• Vector backbone: pCAG-Myc-gw-IP
• Backbone size w/o insert (bp): 6900
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Oct3/4
• Species: M. musculus (mouse)
• Insert Size (bp): 1150
• Mutation: None
• Entrez Gene: Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
• Tag / Fusion Protein:
  • Myc (6x) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CAG-1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry