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Addgene

pCAG-Myc-Oct3/4-IP
(Plasmid #13460)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13460 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCAG-Myc-gw-IP
  • Backbone size w/o insert (bp) 6900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Oct3/4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1150
  • Mutation
    None
  • Entrez Gene
    Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
  • Tag / Fusion Protein
    • Myc (6x) (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-Myc-Oct3/4-IP was a gift from Shinya Yamanaka (Addgene plasmid # 13460 ; http://n2t.net/addgene:13460 ; RRID:Addgene_13460)
  • For your References section:

    Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells. Maruyama M, Ichisaka T, Nakagawa M, Yamanaka S. J Biol Chem. 2005 Jul 1. 280(26):24371-9. 10.1074/jbc.M501423200 PubMed 15863505