pCAG-HA-Sox2-IP
(Plasmid #13459)

• Depositing Lab: Shinya Yamanaka
• Publication: Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9.

Backbone

• Vector backbone: pCAG-HA-gw-IP
• Backbone size w/o insert (bp): 6800
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sox2
• Species: M. musculus (mouse)
• Insert Size (bp): 950
• Entrez Gene: Sox2 (a.k.a. Sox-2, lcc, ysb)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
• 3′ sequencing primer: IRES-R (5'-GCATTCCTTTGGCGAGAG-3')

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag.