Skip to main content
Addgene

actin CP alpha 2 beta 2
(Plasmid #13452)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13452 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pet 24 a
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    capping protein alpha 2 beta 2 (mouse)
  • Alt name
    actin capping protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2000
  • Mutation
    none
  • Entrez Gene
    Capzb (a.k.a. 1700120C01Rik, AI325129, CPB, CPB1, CPB2, CPbeat2, CPbet, CPbeta1, CPbeta2, Cap, Cappb1)
  • Entrez Gene
    Capza2 (a.k.a. 1110053K06Rik, AW208808, Cappa2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco 1 and Nde1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer SEQUENCE
  • 3′ sequencing primer SEQUENCE
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    actin CP alpha 2 beta 2 was a gift from John Cooper (Addgene plasmid # 13452 ; http://n2t.net/addgene:13452 ; RRID:Addgene_13452)

  • For your References section:

    Binding of myotrophin/V-1 to actin-capping protein: implications for how capping protein binds to the filament barbed end. Bhattacharya N, Ghosh S, Sept D, Cooper JA. J Biol Chem. 2006 Oct 13. 281(41):31021-30. 10.1074/jbc.M606278200 PubMed 16895918
Related items:
Commonly requested with: