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Addgene

PGKneotpAlox2
(Plasmid #13444)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13444 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Mammalian Expression, Cre/Lox
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGKneotpAlox2
  • Alt name
    PGKneotpA
  • Insert Size (bp)
    2800

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector contains: phosphoglycerate kinase I (PGK) promoter, neomycin resistance gene (neo), PGK polyA site, and 3x SV40 polyA site (tpA). The PGKneotpA is flanked by loxP sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGKneotpAlox2 was a gift from Philippe Soriano (Addgene plasmid # 13444 ; http://n2t.net/addgene:13444 ; RRID:Addgene_13444)

  • For your References section:

    Generalized lacZ expression with the ROSA26 Cre reporter strain. Soriano P.. Nat Genet. 1999 Jan;21(1):70-1. 10.1038/5007 PubMed 9916792
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