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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13440 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonena
- Backbone size w/o insert (bp) 2900
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePGKdtabpA
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Alt nameDTA
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Insert Size (bp)1600
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector contains: phosphoglycerate kinase I (PGK) promoter, A subunit of the diphtheria toxin gene (dta), bovine growth hormone polyadenylation site (bpA).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PGKdtabpA was a gift from Philippe Soriano (Addgene plasmid # 13440 ; http://n2t.net/addgene:13440 ; RRID:Addgene_13440) -
For your References section:
The PDGF alpha receptor is required for neural crest cell development and for normal patterning of the somites. Soriano P. Development. 1997 Jul . 124(14):2691-700. PubMed 9226440